‘Spain…A Start-Up Nation?’ is the result of a comprehensive research work on the entrepreneurial ecosystem in Spain. It aims to identify the main factors shaping successful entrepreneurial and innovative ecosystems around the globe, and analyse whether they can be adapted to the Spain landscape. With these factors as a starting point, we realise a meticulous study of the situation in Spain, highlighting both positives and negatives. We conclude by spotting potential areas for improvement, as well as a series of implementable measures that may contribute to place Spain at the forefront of entrepreneurship and innovation.